Enzymic Synthesis of 45-O-.BETA.-Galactosyl-maltopentaose by Bacillus circulans .BETA.-Galactosidase.
نویسندگان
چکیده
منابع مشابه
Engineering of the Bacillus circulans β-Galactosidase Product Specificity
Microbial β-galactosidase enzymes are widely used as biocatalysts in industry to produce prebiotic galactooligosaccharides (GOS) from lactose. GOS mixtures are used as beneficial additives in infant formula to mimic the prebiotic effects of human milk oligosaccharides (hMOS). The structural variety in GOS mixtures is significantly lower than in hMOS. Since this structural complexity is consider...
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We report here on the synthesis of nucleotide activated oligosaccharides by transglycosylation with β-galactosidase from Bacillus circulans applying microwave irradiation (MWI) and conventional heating. The presented prod cts could serve as novel inhibitors or donor substrates of Leloir-glycosyltransferases. Some of them have been isolated from human milk but the biological role remains unclear...
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The synthesis of galacto-oligosaccharides (GOS) catalyzed by a novel commercial preparation of β-galactosidase from Bacillus circulans (Biolactase) was studied, and the products were characterized by MS and NMR. Using 400 g/L lactose and 1.5 enzyme units per milliliter, the maximum GOS yield, measured by HPAEC-PAD analysis, was 165 g/L (41% w/w of total carbohydrates in the mixture). The majo...
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Relaxed (relA) mutants of Escherichia coli are defective in beta-galactosidase synthesis during amino acid limitation. We show here that this defect comprises both a transcriptional component and a translational component.
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A novel gene encoding transglycosylating beta-galactosidase (BGase) was cloned from Penicillium expansum F3. The sequence contained a 3,036-bp open reading frame encoding a 1,011-amino-acid protein. This gene was subsequently expressed on the cell surface of Saccharomyces cerevisiae EBY-100 by galactose induction. The BGase-anchored yeast could directly utilize lactose to produce galactooligosa...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1991
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.55.2433